Product information
ELISA Protein C Inhibitor Actibind
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Capture and detecting Antibodies for making an enzyme-immunoassay for quantitative determination of human Protein C Inhibitor activity (PCI) in human plasma.
Test principle
Urokinase-Plasminogen-Activator (u-PA) is incubated in micro wells coated with monoclonal anti-u-PA-antibodies. After binding of the u-PA to the capture antibodies unbound substances are removed by washing. Following this prediluted samples are added and incubated. Hereby u-PA/PCI-complexes are formed. After another washing step a monoclonal peroxidase-conjugated anti-PCI detecting antibody is added and binds to the complex bound PCI. After a washing step added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the Protein C Inhibitor content in the sample.
Reagents and stability (after opening)
| Reagent | Quantity | Stability / note |
|---|---|---|
| Capture antibody | 1 vial | 1 month at 2-8°C |
| Conjugate | 5 vials | 4 hours at RT |
| Standard | 5 vials | 4 hours at RT |
| Urokinase | 5 vials | 4 hours at RT 8 weeks at -70°C |
| Number of determinations | Article number |
|---|---|
| 5 x 96 | TC11100 |