Product information
HaemoMAT®
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The HaemoMAT® assay is an in vitro-assay for the verification of pyrogens as an authorized alternative method to the rabbit pyrogen test.
Test principle
The HaemoMAT® System is based on the stimulation of Peripheral Blood Mononuclear Cells (PBMC). The response of the PBMCs to pyrogenic substances is measured by determination of the amount of released Interleukin-6 (IL-6). IL-6 plays a key role in the immune response reaction of the PBMCs.
To check the suitability of the test system, this kit provides bacterial endotoxin (lipopolysaccharide) as well as three different Non-Endotoxin Pyrogens (NEP) controls.
Assay time: 2 days Handling time Day 1: app. 2 hours Handling time Day 2: app. 3,5 hours
Specifications
| Parameter | Value |
|---|---|
| Numbers of samples | 2 Samples (three dilutions) |
| Non-Endotoxin Pyrogen Controls | 3 |
| IL-6 ELISA Control | 1 |
| Sample Volume | 8 x 20 µl per sample dilution |
| Endotoxin Standard Range | 0,0625 EU/mL- 1 EU/mL |
| Limit of Detection | 0,125 EEU/mL (Endotoxin Equivalent Units) |
Kit components and storage
The MAT-kit consists of two packages that are provided separately under different shipping conditions.
HaemoMAT® Box 1: cool pack (2 – 8°C) HaemoMAT® Box 2: dry ice
| Article number |
|---|
| 31410 |