Product information
ELISA Anti-β2-Glycoprotein I IgG/M/A
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Indirect enzyme linked immunosorbent assays (ELISA) for the quantitative determination of anti-β₂–Glycoprotein I antibodies (isotypes IgG, IgM or IgA) in human serum.
Test principle
Samples, controls and calibrators are incubated in micro wells, which have been coated with β₂–Glycoprotein I, allowing anti β₂–Glycoprotein I antibodies to bind. Unbound substances are removed by washing. An HRP conjugated detecting antibody, specific for human IgG, IgM or IgA is added and binds to the β₂ –Glycoprotein I/ β₂GPI antibody complex. After a washing step, added enzyme substrate reacts with the conjugate. The intensity of the resulting colour is proportional to the content of β₂–Glycoprotein I antibodies in the sample.
Reagents and stability (after opening)
| Reagent | Quantity |
|---|---|
| Micro well strips | 12 x 8 |
| Sample diluent | 1 vial |
| Anti-β₂GPI calibrator serum | 3 vials |
| Anti-β₂GPI positive control | 1 vial |
| Anti-β₂GPI normal control | 1 vial |
| Conjugate | 1 vial |
| Substrate | 1 vial |
| Stopping solution | 1 vial |
| Washing buffer (concentrate) | 2 vials |
When stored at 2-8°C the reagents remain stable until the expiry date printed on the vials.
| Variant | Number of determinations | Article number |
|---|---|---|
| IgG | 96 | 037-001 |
| IgM | 96 | 038-001 |
| IgA | 96 | 039-001 |