Produktinformationen
FXIII Chromogener Assay
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Chromogenic method for quantitative determination of factor XIII-activity in human plasma.
Test principle
Thrombin activates factor XIII to factor XIIIa. Coagulation of the simultaneously formed fibrin is prevented by addition of a fibrin polymerization inhibitor. Activated factor XIII links the amine substrate glycine-ethylene-ester with the glutamine residual of the specific peptide substrate. Ammoniac is released. In the measurement reaction the released ammoniac is demonstrated in a glutamate dehydrogenase catalyzed NADPH dependent reaction. The consumption of NADPH is spectrometrically measured by decrease of extinction at 340 nm. Within one time interval the extinction decrease is proportional to the factor XIII activity.
Reagents
| Reagent | Quantity |
|---|---|
| FXIII Activator Reagent | 3 vial |
| FXIII Detections Reagent | 3 vial |
| FXIII NADPH solution | 3 vials |
| Inhibitor Reagent | 3 vials |
| Stabilizer solution | 1 vial |
Reagent stability after reconstitution
3 days at 2-8°C 3 months at –20°C
Analyzer applications: on request
| Artikelnummer |
|---|
| 5360010 |