Emicizumab calibrator is a calibrator plasma for the calibration of a modified one-step FVIII activity assay for the determination of active emicizumab concentrations in citrated plasma from a clotting time measurement.

Test principle

Emicizumab calibrator enables the quantification of the degree of correction of the APTT-based one-step assay by emicizumab in the sample.

The calibrator is diluted in different concentrations. Each concentration is combined and incubated with FVIII-deficient plasma and APTT reagents. The reaction is started by the addition of CaCl₂. The clotting time is then measured. The correction level of APTT is related to the emicizumab activity and is converted to µg/ml using the calibration curve.

Reagent and stability (reconstituted)

The reagent must be stored unopened at +2-8°C and can be used until the date indicated on the label.